Bodybuilders use beta-alanine as a pre-workout supplement to increase workout efficacy and promote muscle gains. Beta alanine (BA) is a beta amino acid that binds with histidine to form carnosine within muscle tissues.
Carnosine functions in the muscles as a proton buffer, helping to delay the effects of lactic acid build-up. Increasing carnosine levels inside fast twitch (type 2) muscle fibers has been shown to reduce fatigue and improve athletic performance. These results are particularly notable in the 60-240 second range.
Taking beta-alanine for bodybuilding is said to boost carnosine levels more effectively than if you were to consume L-carnosine supplements or powders. It is also used to improve mobility and physical performance in elderly persons.
How does beta-alanine boost gains in lean mass and exercise capacity? This article will examine the physiology of how this supplement works and its potential benefits for bodybuilders.



- Supports muscle growth & workout performance
- Improves athletic stamina & power output
- Increases muscle carnosine levels
Beta Alanine for Bodybuilding
Related Topics
Research shows that using beta alanine supplements to increase muscle carnosine can help bodybuilders in several ways. Carnosine is a small dipeptide which is known primarily for its proton buffering capacities.
In periods of intense physical activity, hydrogen ions are released as by-products of energy metabolism. When hydrogen ions build up in muscle tissue, the muscles begin to “burn”.
Many people erroneously believe that lactic acid accumulations are the cause of muscle burning. It is actually the H+ ions accumulating.
Using beta alanine for bodybuilding helps fortify muscle tissues with carnosine. When hydrogen protons accumulate during strenuous exercise, intramuscular pH levels drop to acidic levels.
Muscular acidosis causes poor performance and loss of power, as well as the “burning” effect. Bodybuilders often want to push past this “burn” to increase muscle tension and promote greater protein synthesis.
Carnosine buffers hydrogen protons from these tissues. Decreasing H+ in muscles prolongs the onset of muscular fatigue. This subsequently is associated with improving endurance, VO2max and certain other performance markers.
By increasing muscle stamina, bodybuilders can work out more effectively at high intensities and complete more repetitions of their lifts. This could accelerate muscle gains.
Carnosine for Bodybuilding
Why take beta-alanine to increase carnosine levels instead of using L-carnosine supplements directly? In research studies, beta-alanine demonstrates a greater potential for increasing muscle carnosine levels because it is more bioavailable.
Carnosine is a dipeptide molecule, formed by the joining of two amino acids. Carnosine is formed by combining beta alanine and histidine; both nonessential aminos.
Research shows that increasing carnosine levels inside the muscles is dependent on the availability of beta alanine; not histidine.
Carnosine can be gained from dietary meats, especially red meats. Vegetarians may develop deficiencies of carnosine over time. Beef, lamb, pork, fowl and fish are all good sources.
However, the amount of carnosine gained from the diet is quickly depleted from the system after ingestion. Using a beta alanine supplement for bodybuilding helps to keep carnosine levels elevated for longer periods of time.
Beta Alanine as a Pre-Workout Supplement
Research supports using beta alanine for enhancing bodybuilding performance. One meta-analysis of BA effects reported a 2.85% median gain in muscular endurance in the 1-3 minute range of training.
HIIT (high intensity interval training) has been proven effective for causing significant aerobic improvements. Oral usage of beta alanine may further increase the benefits of HIIT for bodybuilders.
In one double-blind, human trial involving 46 young men, four 1.5-gram doses of BA were administered daily for three weeks. For the next three weeks, 3-gram doses were given twice daily. All subjects received BA as they engaged in a 6-week HIIT program.
At the study’s conclusion, significant improvements were reported in all of the following:
- VO2peak: volume of oxygen the body can use during exertion;
- VO2TTE: volume of oxygen at time-to-exhaustion during exertion;
- Time to exhaustion (TTE);
- Lean body mass;
Some research also shows that using beta alanine during a cutting phase helps to reduce fat mass. However, this effect is believed to be secondary to increased work capacities and workout volumes.
BA does not seem to have any effect on hormonal responses to physical exertion. Taking 4 grams daily for thirty days has caused no alterations in testosterone, cortisol or human growth hormone.
Although there are no definitive standards set for beta alanine dosing, some bodybuilders recommend using between 3 and 4 grams daily.
Daily supplementation should continue for a minimum of 4 weeks in order to significantly increase intramuscular carnosine stores. Some studies have continued daily administration of BA for up to 10 weeks.
When to Take Beta Alanine for Bodybuilding
Certain pre-workout supplements contain beta alanine, and many bodybuilders use BA just prior to working out. It is also used popularly as an after-workout supplement, believed to help facilitate recovery. However, the effects of BA administration are not time-dependent.
Beta alanine serum levels drop to baseline about three hours after taking an oral BA supplement. For this reason, many users prefer to divide their daily dosages into several smaller doses to be taken every three hours.
Health Benefits of Beta-Alanine and Carnosine
Beta-alanine and Carnosine are becoming popular for several other reasons besides their ability to improve physical performance.
Research suggests that carnosine may also be helpful for:
- Scavenging ROS (reactive oxygen species), RNS (reactive nitrogen species) and other radical agents;
- Suppressing transformation of healthy cells into cancerous cells;
- Inhibiting the release of advanced glycation end products (AGEs);
- Decreasing low-grade systemic inflammation;
- Inhibiting DNA damage;
There has not been sufficient research completed to discern the full scope of carnosine’s health benefits. It demonstrates antiglycation, anticancer, antidiabetic, antiaging, anti-inflammatory, cardioprotective and certain other effects.
Is Using Beta Alanine Safe for Athletes?
The Natural Medicines Comprehensive Database reports that beta alanine is well tolerated in most users. There is a low risk of experiencing irritation in the skin, eyes, respiratory tract and/or digestive tract.
The only side effect experienced commonly is called paresthesia. This condition is marked by skin “crawling”, itching, burning and/or similar sensations.
Paresthesia from BA occurs when too much is taken in one dosage. The paresthesia associated with using beta alanine for bodybuilding is temporary.
It goes away after an hour or so naturally. In most cases, it can be avoided completely by decreasing the daily dosage, or by taking smaller amounts throughout the day as described above.
To be sure that using beta-alanine for bodybuilding is safe for you, speak with a doctor who understands your health history. Let her know about any medications and/or herbal supplements you take.
- Blancquaert L1, Everaert I, Derave W. Beta-alanine supplementation, muscle carnosine and exercise performance. Curr Opin Clin Nutr Metab Care. 2015 Jan;18(1):63-70.
- Derave W1, Everaert I, Beeckman S, Baguet A Muscle carnosine metabolism and beta-alanine supplementation in relation to exercise and training. Sports Med. 2010 Mar 1;40(3):247-63.
- Artioli GG1, Gualano B, Smith A, Stout J, Lancha AH Jr. Role of beta-alanine supplementation on muscle carnosine and exercise performance. Med Sci Sports Exerc. 2010 Jun;42(6):1162-73.
- Lee YT1, Hsu CC, Lin MH, Liu KS, Yin MC. Histidine and carnosine delay diabetic deterioration in mice and protect human low density lipoprotein against oxidation and glycation. Eur J Pharmacol. 2005 Apr 18;513(1-2):145-50. Epub 2005 Apr 2.
- Derave W1, Everaert I, Beeckman S, Baguet A. Muscle carnosine metabolism and beta-alanine supplementation in relation to exercise and training. Sports Med. 2010 Mar 1;40(3):247-63. doi: 10.2165/11530310-000000000-00000.
- Babizhayev MA1, Deyev AI, Yermakova VN, Semiletov YA, Davydova NG, Kurysheva NI, Zhukotskii AV, Goldman IM. N-Acetylcarnosine, a natural histidine-containing dipeptide, as a potent ophthalmic drug in treatment of human cataracts. Peptides. 2001 Jun;22(6):979-94.
- Boldyrev AA [Carnosine metabolism in excitable tissues: biological significance]. Vestn Ross Akad Med Nauk. 1995;(6):3-7.
- Wang AM1, Ma C, Xie ZH, Shen F. Use of carnosine as a natural anti-senescence drug for human beings. Biochemistry (Mosc). 2000 Jul;65(7):869-71.
- Kraemer WJ1, Gordon SE, Lynch JM, Pop ME, Clark KL. Effects of multibuffer supplementation on acid-base balance and 2,3-diphosphoglycerate following repetitive anaerobic exercise. Int J Sport Nutr. 1995 Dec;5(4):300-14.
- Basta G1, Schmidt AM, De Caterina R. Advanced glycation end products and vascular inflammation: implications for accelerated atherosclerosis in diabetes. Cardiovasc Res. 2004 Sep 1;63(4):582-92.
- Beisswenger PJ1, Szwergold BS, Yeo KT. Glycated proteins in diabetes. Clin Lab Med. 2001 Mar;21(1):53-78, vi.
- Bellinger PM1. ?-Alanine supplementation for athletic performance: an update. J Strength Cond Res. 2014 Jun;28(6):1751-70.
- Double-blind, placebo-controlled study of L-carnosine supplementation in children with autistic spectrum disorders. J Child Neurol. 2002 Nov;17(11):833-7.
- Suzuki Y1, Ito O, Mukai N, Takahashi H, Takamatsu K. High level of skeletal muscle carnosine contributes to the latter half of exercise performance during 30-s maximal cycle ergometer sprinting. Jpn J Physiol. 2002 Apr;52(2):199-205.
- Solis MY1, Cooper S2, Hobson RM2, Artioli GG1, Otaduy MC3, Roschel H1, Robertson J2, Martin D2, S Painelli V1, Harris RC4, Gualano B1, Sale C2. Effects of beta-alanine supplementation on brain homocarnosine/carnosine signal and cognitive function: an exploratory study. PLoS One. 2015 Apr 14;10(4):e0123857. doi: 10.1371/journal.pone.0123857. eCollection 2015.
- Marina Yazigi Solis,#1 Simon Cooper,#2 Ruth M Hobson,2 Guilherme G. Artioli,1 Maria C. Otaduy,3 Hamilton Roschel,1 Jacques Robertson,2 Daniel Martin,2 Vitor S. Painelli,1Roger C. Harris,4 Bruno Gualano,1,* and Craig Sale#2,* Effects of Beta-Alanine Supplementation on Brain Homocarnosine/Carnosine Signal and Cognitive Function: An Exploratory Study PLoS One. 2015; 10(4): e0123857. Published online 2015 Apr 14. doi: 10.1371/journal.pone.0123857
- Trexler ET1, Smith-Ryan AE1, Stout JR2, Hoffman JR2, Wilborn CD3, Sale C4, Kreider RB5, Jäger R6, Earnest CP7,Bannock L8, Campbell B9, Kalman D10, Ziegenfuss TN11, Antonio J12. International society of sports nutrition position stand: Beta-Alanine. J Int Soc Sports Nutr. 2015 Jul 15;12:30. doi: 10.1186/s12970-015-0090-y. eCollection 2015.
- Julie Y. Culbertson,1,* Richard B. Kreider,1 Mike Greenwood,2 and Matthew Cooke3 Effects of Beta-Alanine on Muscle Carnosine and Exercise Performance:A Review of the Current Literature Nutrients. 2010 Jan; 2(1): 75–98. Published online 2010 Jan 25. doi: 10.3390/nu2010075
- Lancha Junior AH1, de Salles Painelli V2, Saunders B2, Artioli GG2. Nutritional Strategies to Modulate Intracellular and Extracellular Buffering Capacity During High-Intensity Exercise. Sports Med. 2015 Nov;45 Suppl 1:71-81. doi: 10.1007/s40279-015-0397-5.
- Stegen S1, Stegen B1, Aldini G2, Altomare A2, Cannizzaro L2, Orioli M2, Gerlo S3, Deldicque L4, Ramaekers M4, Hespel P4, Derave W1. Plasma carnosine, but not muscle carnosine, attenuates high-fat diet-induced metabolic stress. Appl Physiol Nutr Metab. 2015 Sep;40(9):868-76. doi: 10.1139/apnm-2015-0042.
- Liu T1, Peng YF, Jia C, Yang BH, Tao X, Li J, Fang X. Ginsenoside Rg3 improves erectile function in streptozotocin-induced diabetic rats. J Sex Med. 2015 Mar;12(3):611-20. doi: 10.1111/jsm.12779. Epub 2014 Dec 1.
- Jordan T1, Lukaszuk J, Misic M, Umoren J. Effect of beta-alanine supplementation on the onset of blood lactate accumulation (OBLA) during treadmill running: Pre/post 2 treatment experimental design. J Int Soc Sports Nutr. 2010 May 19;7:20. doi: 10.1186/1550-2783-7-20.
- Gottesmann C1. GABA mechanisms and sleep. Neuroscience. 2002;111(2):231-9.
- Kern BD1, Robinson TL. Effects of ?-alanine supplementation on performance and body composition in collegiate wrestlers and football players. J Strength Cond Res. 2011 Jul;25(7):1804-15. doi: 10.1519/JSC.0b013e3181e741cf.
- Ulrich P1, Cerami A. Protein glycation, diabetes, and aging. Recent Prog Horm Res. 2001;56:1-21.
- Close GL1, Hamilton L2, Philp A3, Burke L4, Morton JP5. Close GL1, Hamilton L2, Philp A3, Burke L4, Morton JP 5. Free Radic Biol Med. 2016 Feb 4. pii: S0891-5849(16)00030-7. doi: 10.1016/j.freeradbiomed.2016.01.016. [Epub ahead of print]
Article last updated on: March 12th, 2018 by Nootriment